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KMID : 0616120190500010065
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Korean Journal of Pharmacognosy
2019 Volume.50 No. 1 p.65 ~ p.71
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Analysis of the Marker Compounds in Sagunja-tang by LC-ESI-MS
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Seo Chang-Seob
Shin Hyeun-Kyoo
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Abstract
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One of the oriental medicine prescriptions, Sagunja-tang consists of four herbal medicines (Ginseng Radix, Poria Sclerotium, Atractylodis Rhizoma Alba, and Glycyrrhiziae Radix et Rhizoma) and has been used as a medicine to enhance tonify the function of spleen and stomach in Korea. In this study, we conducted simultaneous analysis of the 9 marker components, liquiritin apioside, liquiritin, ginsenoside Rg1, liquiritigenin, ginsenoside Rb1, glycyrrhizin, atractylenolide III, atractylenolide II, and atractylenolide I in Sagunja-tang using a liquid chromatography?electrospray ionization-mass spectrometry (LC-ESI-MS/MS). Marker compounds were separated on a Waters Acquity UPLC BEH C18 analytical column (2.1 × 100 mm, 1.7 mm) and the column was maintained at 45oC. The mobile phase consists of 0.1% (v/v) aqueous formic acid and acetonitrile with gradient condition. The LC-MS analysis was performed using a Waters ACQUITY TQD LC-MS/MS system with multiple reaction monitoring (MRM) method in the positive and negative modes. The calibration curves of the nine marker components showed good linearity with coefficient of determination ³ 0.9984 within tested range. The limits of detection and limits of quantification values were 0.27?2.42 ng/mL and 0.81?7.27 ng/mL, respectively. The concentrations of tested 9 analytes in the lyophilized Sagunja-tang sample using the established LC-ESI-MS/MS MRM method were detected up to 16.593 mg/g. These results can be useful as a basic data for the quality control of an oriental medicine prescriptions.
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KEYWORD
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LC-ESI-MS, Sagunja-tang, Oriental medicine prescription
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